Researchers have engineered the harmless gut bacteria E.coli to generate renewable propane.
The development is a step towards commercial production of a source of fuel that could one day provide an alternative to fossil fuels.
Propane is an appealing source of cleaner fuel because it has an existing global market. It is already produced as a by-product during natural gas processing and petroleum refining, but both are finite resources. In its current form it makes up the bulk of LPG (liquid petroleum gas), which is used in many applications, from central heating to camping stoves and conventional motor vehicles.
In a new study, the team of scientists from Imperial College London and the University of Turku in Finland used Escherichia coli to interrupt the biological process that turns fatty acids into cell membranes. The researchers used enzymes to channel the fatty acids along a different biological pathway, so that the bacteria made engine-ready renewable propane instead of cell membranes.
Their ultimate goal is to insert this engineered system into photosynthetic bacteria, so as to one day directly convert solar energy into chemical fuel.
The results of the study are published in the journal Nature Communications.
Dr Patrik Jones, from the Department of Life Sciences at Imperial College London said: “Although this research is at a very early stage, our proof of concept study provides a method for renewable production of a fuel that previously was only accessible from fossil reserves. Although we have only produced tiny amounts so far, the fuel we have produced is ready to be used in an engine straight away. This opens up possibilities for future sustainable production of renewable fuels that at first could complement, and thereafter replace fossil fuels like diesel, petrol, natural gas and jet fuel.”
The scientists chose to target propane because it can easily escape the cell as a gas, yet requires little energy to transform from its natural gaseous state into a liquid that is easy to transport, store and use.
“Fossil fuels are a finite resource and as our population continues to grow we are going to have to come up with new ways to meet increasing energy demands. It is a substantial challenge, however, to develop a renewable process that is low-cost and economically sustainable. At the moment algae can be used to make biodiesel, but it is not commercially viable as harvesting and processing requires a lot of energy and money. So we chose propane because it can be separated from the natural process with minimal energy and it will be compatible with the existing infrastructure for easy use” added Dr Jones.
Using E. coli as a host organism, the scientists interrupted the biological process that turns fatty acids into cell membranes. By stopping this process at an early stage they could remove butyric acid, a nasty smelling compound that is an essential precursor for propane production.
To interrupt the process, the researchers discovered a new variant of an enzyme called thioesterase which specifically targets fatty acids and releases them from the natural process. They then used a second bacterial enzyme, called CAR, to convert butyric acid into butyraldehyde. Finally, they added a recently discovered enzyme called aldehyde-deformylating oxygenase (ADO), which is known to naturally create hydrocarbons, in order to form propane.
Previous attempts to use the ADO enzyme have proved disappointing as scientists have been unable to harness the natural power of the enzyme to create cleaner fuel. But the scientists at Imperial discovered that by stimulating ADO with electrons they were able to substantially enhance the catalytic capability of the enzyme, and ultimately produce propane.
The level of propane that the scientists produced is currently one thousand times less than what would be needed to turn it into a commercial product, so they are now working on refining their newly designed synthetic process. Dr Jones said: “At the moment, we don’t have a full grasp of exactly how the fuel molecules are made, so we are now trying to find out exactly how this process unfolds. I hope that over the next 5-10 years we will be able to achieve commercially viable processes that will sustainably fuel our energy demands.”
This research was funded by a grant from the European Research Council.
And just so you know
Bacteria (singular ‘bacterium’) are made up of only one simple cell – they are called single-celled organisms.
A bacterium can be shaped like a sphere (coccus), rod (bacillus) or spiral (spirillum).
Bacteria are about 1000 nanometres in size (a nanometre is one millionth of a millimetre).
Different bacteria can live at a huge range of temperatures, from ice to hot springs, and can even live in radioactive waste.
People have more bacterial cells in their bodies than human cells – and more than there are people on the planet.
Most bacteria are useful – gut bacteria produce vitamins and help people (and animals) digest their food, and bacteria in the roots help legumes (plants in the pea and bean family) get nitrogen out of the soil, which helps them to grow.
Bacteria are used in making cheese, yoghurt and sourdough bread (see ‘Make Yoghurt and Grow Yeast’).
Bacteria produce oxygen – perhaps as much as half of the oxygen in the atmosphere.
Bacteria (usually dead or weak ones) are used to make vaccines.
Bacteria are used to clean water in sewage plants.
Bacteria can help clean up oil spills.
Bacterial infections can be cured with antibiotics – drugs that kill bacteria.
Using antibiotics too often, or for diseases that don’t need them, like colds and flu (caused by viruses) can stop the antibiotics working (known as antibiotic resistance). MRSA (methicillin-resistant or Multiple-Resistant Staphylococcus Aureus) is a resistant form of a common bacterium found on the skin, which can cause infections after surgery.
Antibiotics come from fungi.
The above story is based on materials provided by Imperial College London. Note: Materials may be edited for content and length.
Pauli Kallio, András Pásztor, Kati Thiel, M. Kalim Akhtar, Patrik R. Jones. An engineered pathway for the biosynthesis of renewable propane. Nature Communications, 2014; 5: 4731 DOI: 10.1038/NCOMMS5731